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The large quantity of acids produced causes a significant decrease in the pH of the culture medium. MRVP broth pH 6. Methyl red solution, 0. Dissolve 0.
Solution is stable for 1 year. A weak positive is red-orange. Only the addition of reagent differs, and both tests are carried out consecutively.
The development of a stable red color on the surface of the medium indicates sufficient acid production to lower the pH to 4.
Because other organisms may produce smaller quantities of acid from the test substrate, an intermediate orange color between yellow and red may develop.
This does not indicate a positive test. Escherichia coli abbreviated as E. Most strains of E. Classification There are 4 major subgroups and 43 recognized serotypes of […].
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